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Rapid spectral-domain localization
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DOI：10.1364/OE.21.012822.png)
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Abstract
En 中文
We present a method to dynamically image structures at nanometer spatial resolution with far-field instruments. We propose the use of engineered nanoprobes with distinguishable spectral responses and the measurement of coherent scattering, rather than fluorescence. Approaches such as PALM/STORM have relied on the rarity of emission events in time to distinguish signals from distinct probes. By distinguishing signals in the spectral domain, we enable the acquisition of data in a multiplex fashion and thus circumvent the fundamental problem of slow data acquisition of current techniques. The described method has the potential to image dynamic systems with a spatial resolution only limited to the size of the scattering probes. (C) 2013 Optical Society of America
Keywords:
FLUORESCENCE NANOSCOPY
RESOLUTION
COLOCALIZATION
NANOPARTICLES
MICROSCOPY
EMISSION
LIMIT
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