.png){kind=icon}
Return
Ratiometric Barcoding for Mass Cytometry
.png){kind=icon}
OA
AI
DOI：10.1021/acs.analchem.8b03201.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Barcoding is of importance for high-throughput cellular and molecular analysis. A ratiometric barcoding strategy using lanthanide-coordinated polymer dots (Ln-Pdots) was developed for mass cytometric analysis. By using 3 metal isotopes and 4 ratio intensity levels, 16 barcodes were generated to code, and later decode, cell samples in mass cytometry. The ratiometric Ln-Pdot barcodes not only provided high-mass-signal intensities but also eliminated the bias caused by different concentrations of the labeling reagents/barcodes and run-to-run differences in cell labeling efficiency. The ability to distinguish clearly the 16 sets of labeled MCF-7 cells with mass cytometry demonstrated the excellent resolving power of the ratiometric Ln-Pdot barcodes. Furthermore, the results from barcoding PBMC samples via CD45-specific cellular targeting indicated that the ratiometric Ln-Pdot barcodes could facilitate mass cytometry in high-throughput and multiplexed analysis, especially with precious human samples.
Keywords:
SEMICONDUCTING POLYMER DOTS
FLOW-CYTOMETRY
SINGLE CELLS
NANOPARTICLES
MEDICINE
SYSTEM
GUIDE
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
6.7
Papers:
4.7W
Citations:
15.9W
Organization
Cited Papers
Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry.png)
CELL SYSTEMSOAAI
CELL SYSTEMSIF7.7
OAAIShareSaveBeyond model antigens: high-dimensional methods for the analysis of antigen-specific T cellsNATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAIShareSaveShareSaveRapid monoisotopic cisplatin based barcoding for multiplexed mass cytometrySCIENTIFIC REPORTSOAAI
SCIENTIFIC REPORTSIF3.9
OAAIShareSaveShareSavePreparation of uncurled and planar multilayered graphene using polythiophene derivatives via liquid-phase exfoliation of graphiteFlatChemOAAI
FlatChemIF0
OAAIShareSaveCombinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterizationNATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAIShareSave
View more