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Recognition of phospho sugars and nucleotides with an array of boronic acid appended bipyridinium salts
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DOI：10.1016/j.aca.2008.08.011.png)
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Abstract
En 中文
The solution-phase sensor array of three cationic bis-boronic acid appended benzyl viologens (BBV) and the anionic fluorescent dye, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium. salt (HPTS), is able to discriminate among five phospho sugars, four nucleotides and three neutral saccharides in aqueous buffered solution at low mM concentrations. Linear discriminant analysis, principal component analysis, and hierachical cluster analysis studies showed the discrimination limit (lowest analyte concentration where the discrimination is still 100%) to be 4 mM. Calculated K-b and F-max/F-0 values from binding curves of the three BBVs with 1-12 were also used to perform multi-variate analyses with very good discrimination results. (C) 2008 Elsevier B.V. All rights reserved.
Keywords:
Boronic acid
Nucleotide
Phospho sugar
Sensor array
Viologen
Fluorescence
Journal
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6
Papers:
3.3W
Citations:
6.1W
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Cited Papers