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Red fluorescent protein (RFP): An effective reporter gene for Agrobacterium-mediated genetic transformation of Hevea brasiliensis
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DOI：10.1016/j.indcrop.2026.124080.png)
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Abstract
En 中文
• Overcomes a major bottleneck in rubber tree transformation using RFP. • RFP enables real-time, non-destructive tracking of transgenic somatic embryos. • Defines predictable RFP dynamics to eliminate false positives in selection. • WGS confirms stable T-DNA integration, supporting precision breeding of rubber. • Provides a robust toolkit for functional genomics in recalcitrant industrial crops. .
Keywords:
: Red fluorescent protein (RFP)
Hevea brasiliensis
Genetic transformation
Reporter gene
Somatic embryogenesis
Fluorescence dynamics
Molecular breeding
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