Reference-free discovery with barcoded single-cell sequencing

doi:10.1038/s41587-026-03084-6

Reference-free discovery with barcoded single-cell sequencing

2026-04-22

0

PRE

AI

R

Roozbeh Dehghannasiri

Sebastian Deorowicz *

J

Julia Salzman *

DOI：10.1038/s41587-026-03084-6

Original

Original request for help

Save

Abstract

En 中文

Single-cell RNA sequencing (scRNA-seq) analyses typically focus on alignment and differential gene expression, being blind to other transcriptome variation. Here we present sc-SPLASH for statistics-first, reference-free discovery on barcoded scRNA-seq and spatial transcriptomics; its independent BKC submodule optimizes barcoded data preprocessing and is approximately 50-fold faster than UMI-tools. sc-SPLASH discovers secreted repeat proteins in immune-like cells of sponge (Spongilla; missing from the reference) and tunicate (Ciona). sc-SPLASH extends reference-free analysis to barcoded single-cell and spatial transcriptomics data.

Keywords:

Data processing

Genomics

RNA sequencing

Transcriptomics

Life Sciences

general

Biotechnology

Biomedicine

Agriculture

Biomedical Engineering/Biotechnology

Bioinformatics

Reference-free discovery with barcoded single-cell sequencing

Abstract

Journal

Organization