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Revealing species diversity in Pseudo-nitzschia blooms in Scotland and Aotearoa (New Zealand) by combining recombinase polymerase amplification and nanopore-sequencing

delete2026-03-10
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OA
AI
R
Robert G. Hatfield
M
María García-Portela
K
KF Smith
T
Tomohiro Nishimura
S
Sarah Swan
DOI:10.1016/j.hal.2026.103102delete
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Abstract

Abstract

En 中文
• Recombinase polymerase amplification combined with Nanopore sequencing. • Development of assay that provides species resolution of the genus Pseudo-nitzschia. • Assay is applicable to isolates and environmental samples. • Previously undiscovered species found in both Scotland and New Zealand. • RPA lateral flow developed to detect P. australis & P. seriata
Keywords:
RPA
Nanopore sequencing
Harmful algal bloom
HABs
Domoic acid, Amnesic shellfish poisoning
Food safety
Aquaculture
Lateral flow
Pseudo-nitzschia
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Journal

Harmful Algae cover
Harmful Algae
IF:
4.5
Papers:
2.5K
Citations:
9.2K

Organization

I
instituto español de oceanografía and csic
Scholars:
6
Papers: 2
Citations: 0
Scottish Association For Marine Science cover
Scottish Association For Marine Science
Scholars:
38
Papers: 20
Citations: 1.6K
C
Cawthron Institute
Scholars:
562
Papers: 526
Citations: 569
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