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Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction
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DOI：10.1039/c7gc02343j.png)
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Abstract
En 中文
The development of new biocatalysts using ancestral sequence reconstruction is reported. When applied to an omega-transaminase, the ancestral proteins demonstrated novel and superior activities with eighty percent of the forty compounds tested compared to the modern day protein, and improvements in activity of up to twenty fold. These included a range of compounds pertinent as feedstocks in polyamide manufacture.
Keywords:
3-ISOPROPYLMALATE DEHYDROGENASE
OMEGA-TRANSAMINASE
DIRECTED EVOLUTION
ESCHERICHIA-COLI
ACID
PROTEINS
IDENTIFICATION
CHEMISTRY
PATHWAY
ENZYMES
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