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Revisiting biofilms quantification: Crystal Violet Assay and the emerging role of Hyperspectral Imaging
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DOI：10.1039/D6AY00772D.png)
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Abstract
En 中文
Bacterial biofilms are major contributors to persistent and chronic infections. Conventional methods to quantify biofilm; such as the crystal violet assay; provide only semi-quantitative and non-specific estimates of biomass. In this study; we highlight hyperspectral imaging (HSI) as a non-destructive approach for biofilm analysis. To address this; stainless steel coupons were developed to support robust biofilm formation. We propose HSI; coupled with machine learning tools; to detect and quantify Acinetobacter baumannii biofilms for potential real-time monitoring. This method has the potential to capture biochemical as well as structural heterogeneity within biofilms and may serve as a valuable tool in biofilm research.
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