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Rope-skipping for blood plasma separation in point-of-care testing
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DOI：10.1039/D6AN00540C.png)
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Abstract
En 中文
Plasma separation plays an indispensable role in clinical diagnosis and therapeutic applications; since plasma contains critical biomarkers including proteins and nucleic acids; while interference from the color of red blood cells may compromise the accuracy of biomedical detection. Conventional methods; such as centrifugation; are effective but require specialized equipment; skilled personnel; and significant time; making them impractical for point-of-care testing (POCT) in resource-limited settings. The advent of microfluidic plasma separation techniques has successfully addressed the inherent drawbacks of conventional centrifugation approaches. Herein; we propose a rope-skipping microfluidic centrifugation (RSMC) plasma separation method; aiming to better accommodate POCT scenarios. Specifically; a blood-loaded tube was first secured to the skipping-rope device; and the RSMC device was then rotated manually at about 200 rpm speed. Driven by centrifugal force; efficient plasma separation was achieved without the need for electricity or sophisticated instrumentation. Experimental results demonstrated 67.3% plasma yield with 99.99% purity within 5 min; exceeding the performance of existing manual alternatives like fidget-spinner centrifuges. And the plasma separation system can also achieve pre-processing of large volumes of whole blood samples (∼mL). Moreover; the blood glucose recovery rate of plasma reached as high as 98.3% with this method; which was nearly comparable to that obtained using a standard centrifuge. The RSMC method is characterized by its simplicity; high cost-effectiveness; and ease of operation; making it particularly suitable for POCT applications in resource-limited settings.
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