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Salmonella lipopolysaccharide-containing supported lipid bilayers as platforms to study bacteriophage interactions
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DOI：10.1002/1873-3468.70383.png)
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Abstract
En 中文
Lipopolysaccharides (LPS) are a key component in the defensive barrier of the outer membrane (OM) of Gram-negative bacteria; they are a major drug target and bacteriophage access point. Biomimetic platforms presenting LPS are therefore important to study OM biophysics of pathogens and initial bacteriophage infection steps. Herein, we present robust protocols for the preparation of LPS-containing supported lipid bilayers (SLBs) incorporating either Salmonella LPS or OM vesicles (OMVs). We characterized SLBs using quartz crystal microbalance with dissipation (QCM-D) and fluorescence microscopy. We probed their interactions with O-antigen specific Salmonella phages and their tailspike receptor binding proteins. Altogether, this work provides a roadmap to create versatile LPS-based platforms that facilitate studies of interactions between phages and Gram-negative bacterial membranes.
Keywords:
native supported lipid bilayer (nSLB)
Outer membrane vesicle (OMV)
Quartz crystal microbalance with dissipation (QCM-D)
Salmonella Typhimurium
siphovirus
Tailspike protein (TSP)
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