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Saturation variant interpretation using CRISPR prime editing
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DOI：10.1038/s41587-021-01201-1.png)
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Abstract
En 中文
Genetic variants of uncertain significance are characterized with a prime editing method. High-throughput functional characterization of genetic variants in their endogenous locus has so far been possible only with methods that rely on homology-directed repair, which are limited by low editing efficiencies. Here, we adapted CRISPR prime editing for high-throughput variant classification and combined it with a strategy that allows for haploidization of any locus, which simplifies variant interpretation. We demonstrate the utility of saturation prime editing (SPE) by applying it to the NPC intracellular cholesterol transporter 1 gene (NPC1), mutations in which cause the lysosomal storage disorder Niemann-Pick disease type C. Our data suggest that NPC1 is very sensitive to genetic perturbation, with 410 of 706 assayed missense mutations being classified as deleterious, and that the derived function score of variants is reflective of diverse molecular defects. We further applied our approach to the BRCA2 gene, demonstrating that SPE is translatable to other genes with an appropriate cellular assay. In sum, we show that SPE allows for efficient, accurate functional characterization of genetic variants.
Keywords:
NIEMANN-PICK-DISEASE
STEROL-SENSING DOMAIN
LYSOSOMAL STORAGE
BREAST-CANCER
MUTATIONS
PROTEIN
NIEMANN-PICK-C1-DISEASE
FIBROBLASTS
BRCA1
CAS9