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Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging
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DOI：10.1021/acs.nanolett.4c02452.png)
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Abstract
En 中文
Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of similar to 100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.
Keywords:
Photon switch-off effects
Confocal microscope
Exposure dose
Super-resolution microscope
Journal
IF:
9.1
Papers:
2.7W
Citations:
16.5W
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