.png){kind=icon}
.png){kind=icon}
Return
Self-assembling trivalent nanoparticle vaccine enables multivalent antigen display and potent cross-protection against fowl adenovirus serotypes 4, 8b, and 11
.png){kind=icon}
OA
AI
X
W
S
J
K
B
Y
W
L
K
DOI：10.1186/s12951-026-04875-4.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Hepatitis-hydropericardium syndrome, caused by fowl adenovirus serotype 4(FAdV-4), and inclusion body hepatitis, caused by fowl adenovirus 8b and 11 (FAdV-8b and FAdV-11), have become widespread in China, resulting in substantial economic losses. Vaccination remains one of the most effective strategies for disease prevention and control. However, no trivalent subunit vaccine targeting FAdV-4, FAdV-8b, and FAdV-11 has been reported. Therefore, we developed a trivalent nanoparticle vaccine (4-8-11 NPs) by covalently conjugating the fiber knob domains of FAdV-4, FAdV-8b, and FAdV-11 to the surface of Mi3 nanoparticles using the SpyTag/SpyCatcher modular system. The safety and efficacy of the vaccine were evaluated through protein expression analysis, physicochemical characterization, and in vivo immune challenge experiments. The 4-8-11 NPs exhibited high biocompatibility and stability and were preferentially taken up by antigen-presenting cells, thereby promoting their maturation and cytokine production. In vivo studies confirmed that immunization with 4-8-11 NPs induced high titers of neutralizing antibodies and serotype-specific IgG, promoted lymphocyte proliferation, and elicited a balanced Th1/Th2 cytokine response, outperforming the corresponding monomeric antigen. In vivo imaging revealed that 4-8-11 NPs prolonged antigen retention at the injection site and enhanced antigen accumulation in the spleen. Challenge studies demonstrated complete protection against FAdV-4, FAdV-8b, and FAdV-11, with marked reductions in clinical signs, histopathological lesions, and viral load. These findings indicate that the Mi3-based trivalent nanoparticle vaccine elicits robust cross-serotype immune protection and represents a promising strategy for multivalent vaccine development against FAdV.
Keywords:
Fowl adenoviruses
Nanoparticle vaccines
Trivalent vaccines
Multivalent antigen display
Cross-protection
Journal
IF:
12.6
Papers:
5.0K
Citations:
2.8W
Organization
Cited Papers