.png){kind=icon}
Return
Sequential Array Cytometry: Multi-Parameter Imaging with a Single Fluorescent Channel
.png){kind=icon}
OA
AI
DOI：10.1007/s10439-010-0199-8.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Heterogeneity within the human population and within diseased tissues necessitates a personalized medicine approach to diagnostics and the treatment of diseases. Functional assays at the single-cell level can contribute to uncovering heterogeneity and ultimately assist in improved treatment decisions based on the presence of outlier cells. We aim to develop a platform for high-throughput, single-cell-based assays using well-characterized hydrodynamic cell isolation arrays which allow for precise cell and fluid handling. Here, we demonstrate the ability to extract spatial and temporal information about several intracellular components using a single fluorescent channel, eliminating the problem of overlapping fluorescence emission spectra. Integrated with imaging technologies such as wide field-of-view lens-free fluorescent imaging, fiber-optic array scanning technology, and microlens arrays, use of a single fluorescent channel will reduce the cost of reagents and optical components. Specifically, we sequentially stain hydrodynamically trapped cells with three biochemical labels all sharing the same fluorescence excitation and emission spectrum. These markers allow us to analyze the amount of DNA, and compare nucleus-to-cytoplasm ratio, as well as glycosylation of surface proteins. By imaging cells in real-time we enable measurements of temporal localization of cellular components and intracellular reaction kinetics, the latter is used as a measurement of multi-drug resistance. Demonstrating the efficacy of this single-cell analysis platform is the first step in designing and implementing more complete assays, aimed toward improving diagnosis and personalized treatments to complex diseases.
Keywords:
Cytometry
Microfluidics
Single-cell
Immunocytochemistry
High content screening
Cellomics
Drug
discovery
Personalized medicine
Point-of-care
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
5.4
Papers:
6.3K
Citations:
1.4W
Organization
Cited Papers
Fluoxetine inhibits multidrug resistance extrusion pumps and enhances responses to chemotherapy in syngeneic and in human xenograft mouse tumor models.png)
CANCER RESEARCHOAAI
CANCER RESEARCHIF16.6
OAAIShareSaveShareSaveCharacterization of human embryonic stem cell lines by the International Stem Cell InitiativeNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
ShareSaveShareSave
ShareSaveShareSaveSingle-cell enzyme concentrations, kinetics, and inhibition analysis using high-density hydrodynamic cell isolation arraysANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
ShareSaveShareSave
View more