Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics

doi:10.1016/j.bios.2004.02.020

Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics

2004-10-01

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DOI：10.1016/j.bios.2004.02.020

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We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks. (C) 2004 Elsevier B.V. All rights reserved.

Keywords:

silicon microchip

BioMEMS

biosensor

patch-clainp

electrophysiology

biomembranes

Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics

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