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Simple proteomic checks for detecting noncoding RNA
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DOI：10.1002/pmic.200600813.png)
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Abstract
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Proper validation can accelerate sequence-based discovery of proteins and protein-coding genes. Databases currently contain a backlog of experimentally unverified gene models and tentative assignments of observed transcripts to coding or noncoding RNA. We present and apply a general principle, founded on base composition and the genetic code and validated here by bulk 2-D gels, that can improve the reliability of such classifications and of the algorithms or pipelines that lead to them.
Keywords:
2-DE
base composition
isoelectric point
protein predictions
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