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Single Cell Technologies: Beyond Microfluidics

delete2021-07-29
delete15
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OA
AI
H
Haikuo Li
B
Benjamin D. Humphreys *
DOI:10.34067/KID.0001822021delete
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Abstract

Abstract

En 中文
Single-cell RNA-sequencing (scRNA-seq) has been widely adopted in recent years due to standardized protocols and automation, reliability, and standardized bioinformatic pipelines. The most widely adopted platform is the 10x Genomics solution. Although powerful, this system is limited by its high cost, moderate throughput, and the inability to customize due to fixed kit components. This study will cover new approaches that do not rely on microfluidics and thus have low entry costs, are highly customizable, and are within the reach of any laboratory possessing molecular biology expertise.
Keywords:
genetics
basic science
genomics
single-cell analysis
small cytoplasmic RNA

Journal

K
Kidney360
IF:
3
Papers:
1.7K
Citations:
2.5K

Organization

W
washington university (wustl)
Scholars:
5.5W
Papers: 4.5W
Citations: 70
Cited Papers

Cited Papers

Systematic comparison of single-cell and single-nucleus RNA-sequencing methods
err2020-04-06
err538
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errDing, Jiarui; Adiconis, Xian; Simmons, Sean K.; Kowalczyk, Monika S.; Hession, Cynthia C.; Marjanovic, Nemanja D.; Hughes, Travis K.; Wadsworth, Marc H.; Burks, Tyler; Nguyen, Lan T.; Kwon, John Y. H.; Baraks, Boaz; Ge, William; Kedaigle, Amanda J.; Carroll, Shaina; Li, Shuqiang; Hacohen, Nir; Rozenblatt-Rosen, Orit; Shalek, Alex K.; Villani, Alexandra-Chloe; Regev, Aviv; Levin, Joshua Z.
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Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain
err2018-03-28
err436
errOAAI
errRaj, Bushra; Wagner, Daniel E.; McKenna, Aaron; Pandey, Shristi; Klein, Allon M.; Shendure, Jay; Gagnon, James A.; Schier, Alexander F.
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Robust single-cell DNA methylome profiling with snmC-seq2
err2018-09-20
err120
errOAAI
errLuo, Chongyuan; Rivkin, Angeline; Zhou, Jingtian; Sandoval, Justin P.; Kurihara, Laurie; Lucero, Jacinta; Castanon, Rosa; Nery, Joseph R.; Pinto-Duarte, Antonio; Bui, Brian; Fitzpatrick, Conor; O'Connor, Carolyn; Ruga, Seth; Van Eden, Marc E.; Davis, David A.; Mash, Deborah C.; Behrens, M. Margarita; Ecker, Joseph R.
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Single-Nucleus RNA-Seq Is Not Suitable for Detection of Microglial Activation Genes in Humans
err2020-09-01
err182
errOAAI
errThrupp, Nicola; Frigerio, Carlo Sala; Wolfs, Leen; Skene, Nathan G.; Fattorelli, Nicola; Poovathingal, Suresh; Fourne, Yannick; Matthews, Paul M.; Theys, Tom; Mancuso, Renzo; de Strooper, Bart; Fiers, Mark
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Parallel bimodal single-cell sequencing of transcriptome and chromatin accessibility
err2020-07-22
err51
errOAAI
errXing, Qiao Rui; El Farran, Chadi A.; Zeng, Ying Ying; Yi, Yao; Warrier, Tushar; Gautam, Pradeep; Collins, James J.; Xu, Jian; Droge, Peter; Koh, Cheng-Gee; Li, Hu; Zhang, Li-Feng; Loh, Yuin-Han
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Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells
err2016-03-07
err146
errOAAI
errXin, Yurong; Kim, Jinrang; Ni, Min; Wei, Yi; Okamoto, Haruka; Lee, Joseph; Adler, Christina; Cavino, Katie; Murphy, Andrew J.; Yancopoulos, George D.; Lin, Hsin Chieh; Gromada, Jesper
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