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Single Cell Technologies: Beyond Microfluidics
DOI:10.34067/KID.0001822021.png)
Abstract
En 中文
Single-cell RNA-sequencing (scRNA-seq) has been widely adopted in recent years due to standardized protocols and automation, reliability, and standardized bioinformatic pipelines. The most widely adopted platform is the 10x Genomics solution. Although powerful, this system is limited by its high cost, moderate throughput, and the inability to customize due to fixed kit components. This study will cover new approaches that do not rely on microfluidics and thus have low entry costs, are highly customizable, and are within the reach of any laboratory possessing molecular biology expertise.
Keywords:
genetics
basic science
genomics
single-cell analysis
small cytoplasmic RNA
Journal
K
IF:
3
Papers:
1.7K
Citations:
2.5K
Organization
Cited Papers
Systematic comparison of single-cell and single-nucleus RNA-sequencing methods
NATURE BIOTECHNOLOGY
IF41.7
High-throughput sequencing of the transcriptome and chromatin accessibility in the same cell
NATURE BIOTECHNOLOGY
IF41.7
Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain
NATURE BIOTECHNOLOGY
IF41.7
Single-Nucleus RNA-Seq Is Not Suitable for Detection of Microglial Activation Genes in Humans
CELL REPORTS
IF6.9
Parallel bimodal single-cell sequencing of transcriptome and chromatin accessibility
GENOME RESEARCH
IF5.5

