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Single-shot Full-field reflection phase microscopy
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DOI：10.1364/OE.19.007587.png)
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Abstract
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We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/root Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells. (C) 2011 Optical Society of America
Keywords:
RED-BLOOD-CELL
VISCOELASTIC PROPERTIES
MEMBRANE
DYNAMICS
MICRORHEOLOGY
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