Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping

Chungha Lee

doi:10.1364/OE.485559

Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping

2023-04-11

2

OA

AI

DOI：10.1364/OE.485559

Original

Original request for help

Save

Abstract

En 中文

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demon-strated using three-dimensional quantitative phase imaging methods, these methods often require bulky interferometric setups or multiple measurements, which limits the measurement sensitivity and speed. Here, we present a single-shot RI imaging method that visualizes the RI of the in-focus region of a sample. By exploiting spectral multiplexing and optical transfer function engineering, three color-coded intensity images of a sample with three optimized illuminations were simultaneously obtained in a single-shot measurement. The measured intensity images were then deconvoluted to obtain the RI image of the in-focus slice of the sample. As a proof of concept, a setup was built using Fresnel lenses and a liquid-crystal display. For validation purposes, we measured microspheres of known RI and cross-validated the results with simulated results. Various static and highly dynamic biological cells were imaged to demonstrate that the proposed method can conduct single-shot RI slice imaging of biological samples with subcellular resolution.

Keywords:

DIFFRACTION TOMOGRAPHY

CONTRAST

MICROSCOPY

CELLS

QUANTIFICATION

ILLUMINATION

RESOLUTION

Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping

Abstract

Journal

Organization