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Specific interactions between functionalised particles and circulating tumour cells
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DOI：10.1049/iet-nbt.2013.0034.png)
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Abstract
En 中文
Receptor-ligand binding has been one of the more popular approaches to specifically targeting tumour cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM-expressing BT-20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalised particles bound to target cells were experimentally investigated. The number of bound particles was found to increase with particle concentration, but not necessarily with incubation time. Binding affinity loss because of cell-particle-cell interaction was identified as a limiting mechanism for the number of particles bound to target cells. While cell-surface coverage because of bound particles rises exponentially under low particle concentration, it features a peak value at high particle concentration. The current findings suggest that separation of a bound particle from a cell may be detrimental to cellular binding affinity.
Keywords:
cellular biophysics
silicon compounds
tumours
functionalised particle interactions
circulating tumour cells
receptoraEuroligand binding
silica particles
EpCAM antibodies
EpCAM-expressing BT-20 breast cancer cells
incubation time
particle concentration
functionalised particles
bound particles
binding affinity loss
cellaEuroparticleaEurocell interaction
cell-surface coverage
cellular binding affinity
SiO2