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Strategies for enhancing tocopherol enrichment based on strain screening, optimization, and regulation
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DOI：10.1016/j.jff.2026.107391.png)
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Abstract
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• Monascus. rubiginosus (M. rbg) exhibited the greatest total tocopherol accumulation ability among the four Monascus spp. during coix seed fermentation. • The optimization strain (M. rbg-2 M) with a 2.98-fold increase in total tocopherol yield compared to the initial strain (M. rbg). • Supplementation with key regulation factors—L-Phenylalanine (L-Phe), L-Tyrosine (L-Tyr), and sodium dodecyl sulfate (SDS) significantly increased both total tocopherol enrichment and the diversity of tocopherol isomers. • The synergistic combination of strain optimization and fermentation regulation elevated total tocopherol in the M. rbg-2 M to 346.49 μg/g, over fifteen-fold higher than the M. rbg.
Keywords:
Tocopherol
Monascus spp
Solid-state fermentation
Enhancement
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