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Super-resolution microscopy demystified

delete2019-01-02
delete806
PRE
AI
L
Lothar Schermelleh *
A
Alexia Ferrand
T
Thomas Huser
C
Christian Eggeling
M
Markus Sauer
O
Oliver Biehlmaier
G
Gregor P. C. Drummen *
DOI：10.1038/s41556-018-0251-8delete
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Abstract

Abstract

En 中文
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
Keywords:
STRUCTURED-ILLUMINATION MICROSCOPY
RESOLUTION FLUORESCENCE MICROSCOPY
EXPANSION MICROSCOPY
LIVE CELLS
LOCALIZATION MICROSCOPY
CELLULAR STRUCTURES
DIFFRACTION LIMIT
IMAGE-RESOLUTION
STED NANOSCOPY
DNA ORIGAMI
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AI Summary

Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

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Nature Cell Biology cover
Nature Cell Biology
IF:
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Citations:
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