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Suspended Tissue Open Microfluidic Patterning (STOMP)

delete2025-04-29
delete1
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OA
AI
A
Amanda J. Haack
L
Lauren G. Brown
P
Priti Mulimani
J
Jean Berthier
A
Asha R. Viswanathan
I
Irina Kopyeva
J
Jamison M. Whitten
A
Ariel Lin
S
Serena H. Nguyen
T
Thomas Leahy
E
Ella E. Bouker
R
Ruby M. Padgett
N
Natalie A. Mazzawi
J
Jodie C. Tokihiro
R
Ross C. Bretherton
A
Aaliyah Wu
S
Stephen J. Tapscott
C
Cole A. DeForest
T
Tracy E. Popowics
E
Erwin Berthier
N
Nathan J. Sniadecki
A
Ashleigh B. Theberge
DOI：10.1002/advs.202501148delete
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Abstract

Abstract

En 中文
Free-standing tissue structures tethered between pillars are powerful mechanobiology tools for studying cell contraction. To model interfaces ubiquitous in natural tissues and upgrade existing single-region suspended constructs, we developed Suspended Tissue Open Microfluidic Patterning (STOMP), a method to create multi-regional suspended tissues. STOMP uses open microfluidics and capillary pinning to pattern subregions within free-standing tissues, facilitating the study of complex tissue interfaces, such as diseased-healthy boundaries (e.g., fibrotic-healthy) and tissue-type interfaces (e.g., bone-ligament). We observed altered contractile dynamics in fibrotic-healthy engineered heart tissues compared to single-region tissues and differing contractility in bone-ligament enthesis constructs compared to single-tissue periodontal ligament models. STOMP is a versatile platform - surface tension-driven patterning removes material requirements common with other patterning methods (e.g., shear-thinning, photopolymerizable) allowing tissue generation in multiple geometries with native extracellular matrices and advanced four-dimensional (4D)- materials. STOMP combines the contractile functionality of suspended tissues with precise patterning, enabling dynamic and spatially controlled studies.
Keywords:
hydrogel patterning
open microfluidics
suspended tissue
tissue engineering
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Journal

Advanced Science cover
Advanced Science
IF:
14.1
Papers:
1.7W
Citations:
11.5W

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