Synthetic spatial aperture holographic third harmonic generation microscopy

Yusef Farah

doi:10.1364/OPTICA.521088

Synthetic spatial aperture holographic third harmonic generation microscopy

2024-05-15

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AI

DOI：10.1364/OPTICA.521088

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Third harmonic generation (THG) provides a valuable, label-free approach to imaging biological systems. To date, THG microscopy has been performed using point-scanning methods that rely on intensity measurements lacking phase information of the complex field. We report the first demonstration, to the best of our knowledge, of THG holographic microscopy and the reconstruction of the complex THG signal field with spatial synthetic aperture imaging. Phase distortions arising from measurement-to-measurement fluctuations and imaging components cause optical aberrations in the reconstructed THG field. We have developed an aberration-correction algorithm that estimates and corrects these phase distortions to reconstruct the spatial synthetic aperture THG field without optical aberrations. (c) 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

Keywords:

3RD-HARMONIC GENERATION

RESONANCE ENHANCEMENT

ADAPTIVE OPTICS

SPECTROSCOPY

CELLS

Synthetic spatial aperture holographic third harmonic generation microscopy

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