.png){kind=icon}
.png){kind=icon}
Return
TdT/Cas12a Cascade Amplification Biosensor for Sensitive ALP Activity Detection
.png){kind=icon}
PRE
AI
DOI：10.1039/D5AN00938C.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
This study presents a novel biosensor based on TdT and CRISPR-Cas12a; which integrates the catalytic activity of terminal deoxynucleotidyl transferase (TdT) with the trans-cleavage property of CRISPR-Cas12a to achieve ultra-sensitive biomolecular detection. The biosensor exhibited a broad linear detection range from 0 to 0.2 U/L and a remarkably low detection limit of 1.7×10⁻³ U/L; demonstrating high specificity and sensitivity. In practical validation; the biosensor successfully quantified alkaline phosphatase (ALP) activity in both cervical cancer cells and HeLa cell lysates; even at a dilution factor of up to 10⁶-fold. Its sensitivity allowed precise detection at the single-cell level. This technology offers a robust; simple; and cost-effective platform for cancer diagnosis; treatment monitoring; and enzyme inhibitor screening; while maintaining excellent detection performance in complex biological samples. This breakthrough establishes a foundation for serological tumor screening and early disease diagnosis; while also opening new avenues for enhanced cancer management and clinical translation; indicating significant potential in translational medicine.