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Temperature Range Thermal Proteome Profiling for Drug Target Identification: A Practical Guide
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DOI：10.1021/acs.jproteome.5c01009.png)
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Abstract
En 中文
Determining the drug targets and off-targets is crucial and can provide key information for the drug action mechanism. As one of the multiple methods used to identify the drug targets, thermal proteome profiling (TPP) is more direct, simpler, and suitable for large-scale applications. TPP can also be used at the live-cell level to obtain more information about the pathway regulation beyond the direct targets. At present, there are three formats of TPP: temperature range TPP (TPP-TR), compound concentration range TPP (TPP-CCR), and two-dimensional TPP (2D-TPP). TPP-TR is the most widely practiced form and has been improved in different aspects. In this review, we systematically discuss the evolution, advantages, and limitations of TPP-TR sample preparation and data analysis to provide potential strategies for the development and application of innovative methods.
Keywords:
Peptides and proteins
Proteomics
Sample preparation
Thermal stability
Thermodynamic properties
drug discovery
proteomics
target protein
thermal proteome profiling
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