.png){kind=icon}
.png){kind=icon}
Return
The application of CRISPR gene-editing technology in influenza prevention and control
.png){kind=icon}
OA
AI
X
H
J
L
X
X
DOI：10.3389/fgeed.2026.1844919.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Influenza A virus (IAV) and influenza B virus (IBV) remain major global public health threats because of their rapid antigenic evolution and efficient human-to-human transmission. In contrast; influenza C virus (ICV) and influenza D virus (IDV) generally exhibit narrower host ranges and milder pathogenicity; yet their potential for interspecies transmission and zoonotic spillover still warrants attention. Conventional prevention strategies; such as inactivated and live-attenuated vaccines; suffer from prolonged development timelines and diminished efficacy against rapidly evolving viral strains. However; antiviral drugs are increasingly limited by the rapid emergence of drug-resistant variants. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) gene-editing technology has emerged as a promising platform for influenza prevention and control owing to its programmability and precise targeting capability. In this paper; we summarize recent advances in CRISPR-based strategies for influenza prevention and control. The RNA-targeting CRISPR-associated protein 13 (Cas13) system can recognize conserved viral RNA sequences and suppress replication across influenza subtypes; whereas the DNA-targeting CRISPR-associated protein 9 (Cas9) system can edit host susceptibility genes and thereby reduce cellular permissiveness to infection. In addition; lipid nanoparticle (LNP)-based delivery systems have become important tools for improving the in vivo delivery and expression of CRISPR components by enhancing targeting efficiency and reducing immunogenicity. CRISPR-based diagnostics; such as Specific High-sensitivity Enzymatic Reporter unLOCKing (SHERLOCK); further expand the clinical utility of this technology by enabling rapid and sensitive detection of influenza viruses. Despite these advances; substantial challenges remain; including delivery inefficiency; off-target activity; long-term safety concerns; and the risk of viral escape. With continued technological refinement and careful translational development; CRISPR may become a versatile tool for influenza prevention; diagnosis; and therapy.
Keywords:
influenza B virus
influenza a virus
lipid nanoparticle delivery
crispr/cas gene editing
host susceptibility gene editing
viral RNA targeting
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
F
IF:
4.4
Papers:
82
Citations:
893
Organization
Cited Papers