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The effects of suppressor of cytokine signalling 3 in glucose intolerance of Japanese flounder (Paralichthys olivaceus)
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DOI：10.1017/S0007114525105321.png)
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Abstract
En 中文
To explore molecular targets for regulating glucose metabolism in carnivorous fish; the glucose tolerance test was carried out on the Paralichthys olivaceus. The concentration of glucose and insulin in serum was measured at 0; 1; 3; 5; 7; 9; 12; 24 and 48 h after intraperitoneal (IP) injection. The concentration of insulin was the lowest after 3 h of glucose injection; and that of glucose reached the highest after 5 h. Therefore; 0 h (IP0) was chosen as control group; and 3 h (IP3) and 5 h (IP5) were selected as experimental groups; for which the liver samples at three time points were used for high-throughput sequencing. Although there were no significant Kyoto Encyclopedia of Genes and Genomes and Gene Ontology functional enrichment; the differential genes; including mitogen-activated protein kinase binding protein 1; glycosyltransferase; suppressor of cytokine signalling 3 (SOCS3) and CCAAT/enhancer-binding protein α; were closely related to glucose metabolism. The open reading frame of SOCS3 encoded 225 amino acids; including conserved domains SH2 and SOCS3-box. The SOCS3 was knocked down by specific siRNA in the primary hepatocyte of P. olivaceus. Results showed that the gene expression of insulin receptor substrate 1; protease B1; GLUT2; pyruvate kinase and glucokinase increased significantly after knocking down SOCS3. Meanwhile; the phosphatidylinositol-3-hydroxykinase and glucose-6-phosphatase decreased significantly. The results of this study indicated that siSOSC3 enhanced the sensitivity of the insulin signalling pathway to promote glucose transport; thereby affecting gluconeogenesis and glycolysis to maintain glucose homeostasis.
Keywords:
Paralichthys olivaceus
Glucose tolerance test
Transcriptomics
SOSC3
complementary DNA
γ-glutamyltransferase
Gene Ontology
glucose tolerance test
intraperitoneal
phosphatidyl Inositol 3-kinase
suppressor of cytokine signalling 3
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3
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