The ISChip: A high-throughput qPCR array for absolute quantification of insertion sequences across the One Health continuum

doi:10.1016/j.envpol.2026.128649

The ISChip: A high-throughput qPCR array for absolute quantification of insertion sequences across the One Health continuum

2026-06-25

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DOI：10.1016/j.envpol.2026.128649

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• ISChip enables absolute quantification of 183 insertion sequences (IS). • Profiling 69 samples across the One Health continuum identifies major IS hotspots. • Anthropogenic inputs drive IS accumulation across the water continuum. • A conserved, high-abundance core IS assemblage persists in human gut.

The ISChip: A high-throughput qPCR array for absolute quantification of insertion sequences across the One Health continuum

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