Tissue-specific metabolite profiling reveals diverse metabolic response under N deficiency and high temperature stress in soybean

2026-08-07

Hamza Tariq

Muhammad Saqib Bilal

Lei Wang

Alisdair R. Fernie

Mohsin Tanveer *

DOI：10.1016/j.jare.2026.08.017

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Abstract

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• Compound heat and nitrogen deficiency cause synergistic damage. • Roots and shoots exhibit distinct, complementary metabolic strategies. • Roots prioritise nitrogen assimilation, while leaves enhance antioxidant flavonoids and osmoprotection. • Machine learning identifies key metabolite hubs predicting resilience. • WGCNA revealed diverse metabolic signatures beyond single stresses.

Keywords:

Soybean

Combined stress

Metabolomics

Tissue-specific response

Machine learning

N metabolism

Metabolic reprogramming

Tissue-specific metabolite profiling reveals diverse metabolic response under N deficiency and high temperature stress in soybean

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