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Toward Minimally Invasive Metabolomics: GC-MS metabolic fingerprints of Dried Blood Microsamples in comparison to Plasma
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DOI：10.1039/D5AN00937E.png)
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Abstract
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Global untargeted profiles of dried blood microsamples (BμS) were studied in comparison to conventional plasma and blood samples using Gas Chromatography-Mass Spectrometry (GC-MS). Venous blood from 10 healthy; overnight-fasted individuals was collected and used to produce dried microsamples on Whatman cards; Capitainer and Mitra devices. In parallel paired plasma samples were collected. The metabolite extraction protocol was optimized and methanol was selected as the extraction solvent; while methoximation and trimethylsilylation derivatization was applied. BµS; mainly from Mitra and Capitainer devices; provided equivalent or greater information than plasma; considering the number and intensity of features; precision; and annotated metabolites intensity. Furthermore; blood microsample metabolic profiles collected from the fingertip of three individuals were compared against paired plasma and blood. Overall; the results suggest that BμS is a viable alternative for untargeted blood metabolomics; providing comparable information. Capillary blood collected using Mitra devices exhibits variations when compared to venous whole blood and plasma; with distinct trends observed across several metabolites. Consequently; further research is necessary to determine the applicability of this approach in specific contexts.
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