logo
CanyamAI summaries for academic research
language
MobileMobile
  • Phone FeatureWeChat Mini Program
EnglishEnglish
noticeNotice
BlogBlog
delete
arrow
Return

Transfection Potency of Lipid Nanoparticles Containing mRNA Depends on Relative Loading Levels

delete2024-12-31
delete0
delete
OA
AI
S
Suiyang Liao
A
Abishek Wadhwa
A
Alexandra Birkenshaw
K
Kevin Fox
M
Miffy H. Y. Cheng
I
Irafasha C. Casmil
A
Armando Alcázar Magaña
N
Nuthan Vikas Bathula
C
Chia-Hao Ho
程俊 cover
程俊 (J. Cheng)
L
Leonard J. Foster
K
Kenneth W. Harder
C
Colin J.D. Ross
P
Pieter R. Cullis *
A
Anna K. Blakney *
DOI：10.1021/acsami.4c20077delete
deleteOriginal
deleteShare
deleteSave
View PDF
Abstract

Abstract

En 中文
When formulating mRNA into lipid nanoparticles (LNP), various copy numbers of mRNA are encapsulated, leading to a distribution of mRNA loading levels within the LNPs. It is unclear whether the mRNA loading level affects the functional delivery of the message. Here we show that depending on the mRNA loading level, LNPs exhibit distinct mass densities and can be fractionated via ultracentrifugation. Upon fractionation, we investigated if mRNA loading levels influence LNP sizing, lipid composition, and morphology. We further conducted in vitro and in vivo functional delivery of mRNA and found that the LNP fraction with the highest mRNA loading levels was the least transfection competent.
Keywords:
lipid nanoparticles
mRNA
therapeutics
encapsulation
fractionation
AI Summary

AI Summary

Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

ACS Applied Materials and Interfaces cover
ACS Applied Materials and Interfaces
IF:
8.2
Papers:
6.1W
Citations:
38.7W

Organization

No organization information available