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Trophic state modulates congruence between metabarcoding and microscopy in freshwater biomonitoring

delete2026-08-06
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OA
AI
M
Manuel Castro Berman *
A
Allison R. Hrycik
E
Emily Palmer
J
Jeffrey R. Johansen
K
Kaitlin L. Reinl
J
Jonathan S. Dordick
K
Kevin C. Rose
B
Ben T. Larson
DOI：10.1016/j.ecolind.2026.115301delete
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Abstract

Abstract

En 中文
• eDNA recovers higher phytoplankton diversity than microscopy across trophic gradients. • Microscopy diversity saturates under hypereutrophic conditions metabarcoding does not. • 16S rRNA reveals clearer communities' structures than microscopy and 18S rRNA. • Metabarcoding detects diverse microorganisms that are undetected by light microscopy.
Keywords:
eDNA metabarcoding
Phytoplankton
Bacteria
Light microscopy
Trophic gradient
Long-read sequencing
Chloroplast

Journal

Ecological Indicators cover
Ecological Indicators
IF:
7.4
Papers:
1.3W
Citations:
7.1W

Organization

U
university heights
Scholars:
41
Papers: 16
Citations: 2
E
enviroscience inc.
Scholars:
2
Papers: 1
Citations: 0
R
rensselaer polytechnic institute
Scholars:
7.0K
Papers: 6.5K
Citations: 6
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