Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation

Ailion, Michael

doi:10.1016/j.neuron.2014.02.017

Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation

2014-04-01

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DOI：10.1016/j.neuron.2014.02.017

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Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network.

Keywords:

IMMATURE SECRETORY GRANULES

GTP-BINDING-PROTEINS

CAENORHABDITIS-ELEGANS

C-ELEGANS

RUN DOMAIN

RAB6-INTERACTING PROTEIN-1

HOMOTYPIC FUSION

TRANS-GOLGI

UNC-31 CAPS

PC12 CELLS

Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation

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