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Unlocking Species and Subspecies Diversity of Harmful Microalgae Using HiFi Long-Read Metabarcoding: Insights from Amphidomataceae (Dinophyceae) in the China Sea
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DOI：10.1016/j.hal.2026.103103.png)
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Abstract
En 中文
• Reproducible HiFi long-read workflow achieving species/ribotype resolution for harmful microalgae. • ITS–LSU HiFi survey recovered 44 lineages (17 known, 27 putative) along China’s coast, greatly extending recognized Amphidomataceae diversity. • Basin-specific AZAs contributors: Am. languida and Az. poporum (Bohai/Yellow); Az. dexteroporum, Az. poporum, and Az. spinosum (East/South China). • Niche metrics (Levins’ B², Schoener’s D) and k-means (SST–salinity–TRIX) identified three ecological groups that match MaxEnt habitat suitability for AZAs producers.
Keywords:
Harmful microalgae
HiFi long-read metabarcoding
Amphidomataceae
Species diversity
Ecological groups
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