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Unraveling the Mechanism of Ainsliaea fragrans Champ. In Alleviating Cervicitis via the AMPK-mTOR-HIF-1α Signaling Axis
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DOI：10.1177/1934578X261445198.png)
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Abstract
En 中文
BackgroundCervicitis is a common gynecological disorder with high recurrence and poorly understood mechanisms. Ainsliaea fragrans Champ. (AF) is used in traditional medicine for treating cervicitis, yet its active components and mechanism remain unclear.Aim of the StudyThis study aims to identify the anti-cervicitis active components of AF, and to evaluate its possible mechanism of action.MethodsThe chemical constituents absorbed into serum were identified using UPLC-Q-Exactive-Orbitrap-MS. Network pharmacology was applied to predict targets and pathways, and a rat cervicitis model was established for pharmacological evaluation, including histopathological examination, inflammatory cytokine assays, and cervical transcriptome sequencing. Key signaling pathways were further validated by western blotting.Results31 AF-derived components were detected in the serum. AF treatment ameliorated weight loss, reduced the vaginal index, decreased serum levels of IL-1 beta, IL-6, and TNF-alpha, and alleviated histopathological damage in cervicitis rats. Integrated analysis of network pharmacology and transcriptomics suggested the involvement of the AMPK-mTOR-HIF-1 alpha signaling axis. Molecular docking indicated favorable binding affinities between key candidate components (e.g., glycyrrhizin, sanggenone H) and core targets (STAT3, MTOR, HIF-1 alpha, AMPK). Western blot analysis confirmed that AF treatment upregulated p-AMPK expression while downregulating p-mTOR and HIF-1 alpha protein levels.ConclusionThe findings suggest that the improvement of cervicitis inflammation and injury by AF is associated with the modulation of the AMPK-mTOR-HIF-1 alpha signaling axis, and this integrated strategy provides a mechanistic basis for the anti-cervicitis effect of AF, supporting its further investigation as a potential therapeutic agent.
Keywords:
Ainsliaea fragrans champ.
cervicitis
network pharmacology
transcriptomics
AMPK
mTOR
HIF-1 alpha
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