.png){kind=icon}
.png){kind=icon}
Return
Unusual organization, complexity and redundancy at the Escherichia coli hcp-hcr operon promoter
.png){kind=icon}
OA
AI
D
D
G
S
DOI：10.1042/BJ20100623.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Expression from the Escherichia coli hcp-hcr operon promoter is optimally induced during anaerobic conditions in the presence of nitrite. This expression depends on transcription activation by FNR (fumarate and nitrate reduction regulator), which binds to a target centred at position -72.5 upstream of the transcript start site. Mutational analysis was exploited to identify the corresponding 10 and 35 hexamer elements. A DNA site for NarL and NarP, located at position -104.5, plays only a minor role, whereas NsrR binding to a DNA target centred at position +6 plays a major role in induction of the hcp-hcr operon promoter. Electrophoretic mobility-shift assays show that NsrR binds to this target. The consequences of this for the kinetics of induction of the hcp-hcr operon are discussed.
Keywords:
Escherichia coli
NarL
NsrR
nitrate
nitrite
promoter
reactive nitrogen species
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
4.3
Papers:
1.5W
Citations:
3.8W
Organization
Cited Papers