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User-friendly image-activated microfluidic cell sorting technique using an optimized, fast deep learning algorithm
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AI
DOI：10.1039/d0lc00747a.png)
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Abstract
En 中文
Image-activated cell sorting is an essential biomedical research technique for understanding the unique characteristics of single cells. Deep learning algorithms can be used to extract hidden cell features from high-content image information to enable the discrimination of cell-to-cell differences in image-activated cell sorters. However, such systems are challenging to implement from a technical perspective due to the advanced imaging and sorting requirements and the long processing times of deep learning algorithms. Here, we introduce a user-friendly image-activated microfluidic sorting technique based on a fast deep learning model under the TensorRT framework to enable sorting decisions within 3 ms. The proposed sorter employs a significantly simplified operational procedure based on the use of a syringe connected to a piezoelectric actuator. The sorter has a 2.5 ms latency. The utility of the sorter was demonstrated through real-time sorting of fluorescent polystyrene beads and cells. The sorter achieved 98.0%, 95.1%, and 94.2% sorting purities for 15 mu m and 10 mu m beads, HL-60 and Jurkat cells, and HL-60 and K562 cells, respectively, with a throughput of up to 82.8 events per second (eps).
Keywords:
SINGLE-CELL
GENE-EXPRESSION
FLOW-CYTOMETRY
CHIP