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Versatile Capillary Column Temperature Control Using a Thermoelectric Array Based Platform
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DOI：10.1021/ac2004955.png)
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Abstract
En 中文
A new direct contact platform for capillary column precise temperature control based upon the use of individually controlled sequentially aligned Peltier thermoelectric units is presented. The platform provides rapid temperature control for capillary and microbore liquid chromatography columns and allows simultaneous temporal and spatial temperature programming. The operating temperature range of the platform was between 15 and 200 degrees C for each of 10 aligned Peltier units, with a ramp rate of approximately 400 degrees C/min. The system was evaluated for a number of nonstandard capillary based applications, such as the direct application of temperature gradients with both linear and nonlinear profiles, including both static column temperature gradients and temporal temperature gradients, and the formation of in-capillary monolithic stationary phases with gradient polymerization through precise temperature control.
Keywords:
PERFORMANCE LIQUID-CHROMATOGRAPHY
GRADIENT ELUTION
PHASE
WATER
SELECTIVITY
SEPARATION
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