Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe

doi:10.1126/sciadv.adz6633

Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe

2025-10-22

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DOI：10.1126/sciadv.adz6633

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Early detection and late-stage cell fate assessment are key factors to develop therapeutic strategies, although current methods cannot capture early responses or distinguish multiple injury states, especially in ultraviolet-visible (UV-vis)–sensitive cells. Here, we introduce a method to simultaneously detect variations in RNA and DNA under near-infrared photoexcitation. Using a pyrazinacene-based probe (TEG8-N14), we unexpectedly achieved discrimination of multiple cell states, including apoptosis, necrosis, necroptosis, and senescence, based on RNA/DNA changes. Specifically, TEG8-N14 selectively stains necrotic cells in live samples, while after fixation, it allows detection of ultraearly senescence in UV-vis–sensitive cells, providing approximately twofold greater informational content than existing RNA or DNA fluorophores. These findings break current imaging barriers by enabling comprehensive visualization of single-cell fate histories without being affected by UV-vis or genetic manipulation.

Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe

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