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Volumetric localization microscopy with deep learning
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DOI：10.1038/s41467-025-65941-3.png)
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Abstract
En 中文
Super-resolution microscopy, particularly localization-based methods, necessitates careful balancing of optical complexity, computational demands, and user accessibility. Conventional strategies typically adopt either deterministic or learning-based approaches, overlooking opportunities to leverage their synergistic strengths. In this work, we introduce volumetric localization microscopy (VLM) with deep learning, a super-resolution methodology that integrates instrumental and algorithmic advancements for high-fidelity 3D single-molecule imaging. VLM employs a wavefront-optimized light-field configuration to capture single-molecule data, while a cascaded neural network reconstructs 3D volumes and extracts molecular coordinates at a 10 nm lateral and 25 nm axial localization precision with effective imaging depth over 4 µm. Unlike existing methods, VLM is trained exclusively with system-aware intrinsic point-spread functions, bypassing dependencies on external imaging modalities or sample-specific data training. We validate VLM across diverse biological specimens, demonstrating hardware simplicity, data efficiency, and minimal phototoxicity. We anticipate VLM will overcome current limitations in fluorescence microscopy, empowering broader advancements in biomedical research. Volumetric Localization Microscopy (VLM) integrates light-field imaging with deep learning for high-fidelity 3D single-molecule imaging. Trained on system-aware PSFs, VLM offers simple, efficient, low-toxicity 3D imaging for biomedical research.
Keywords:
Volumetric Localization Microscopy
Deep Learning
Super-resolution Microscopy
Single-molecule Imaging
Light-field Imaging
Journal
IF:
15.7
Papers:
9.3W
Citations:
91.2W