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Whole Genome Features and Analysis of Antibiotic Resistance Determinants in Pseudomonas aeruginosa Strain CYZ
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DOI：10.1177/10766294251388935.png)
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Abstract
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Background: Pseudomonas aeruginosa is an opportunistic pathogen that exhibits a strong resistance ability to antibiotics.Methods: The complete genome of P. aeruginosa CYZ was sequenced using a PacBio RS II system. The functions of all the predicted genes and proteins were classified and annotated using the Clusters of Orthologous Groups of proteins, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases. Furthermore, the antimicrobial resistance genes were also analyzed via the Comprehensive Antibiotic Resistance Database, and several types of antibiotics were selected to test the antimicrobial susceptibility. Phylogenetic relationships were investigated using the single nucleotide polymorphisms (SNPs) from 228 clinically isolated P. aeruginosa strains.Results: The genome size of P. aeruginosa CYZ is 6,382,603 bp and contains 5,807 protein-coding genes, with an average G + C content of 66.44%. Functional genomic analysis via the annotations of the databases identified a total of 499 antimicrobial resistance genes. P. aeruginosa CYZ showed resistance to 14 types of antibiotics. The phylogenetic analysis revealed that P. aeruginosa CYZ was closely related to P. aeruginosa PAO1. The P. aeruginosa strains exhibited no geographical specificity, and the variation in core genome SNPs was nonrandomly distributed.Conclusion: Our findings give valuable insight into the genetic antimicrobial-resistant features of P. aeruginosa as well as provide a genetic basis for the further study of the phenotype.
Keywords:
Pseudomonas aeruginosa
complete genome sequence
multidrug resistance
phylogenetic analysis
SNP analysis
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