A practical guide to studying genome function using single-molecule genomics

Arnaud Krebs

doi:10.1038/s41580-026-01017-4

2026-09-14

PRE

Arnaud Krebs *

Nicolas Altemose *

L. Stirling Churchman *

William J. Greenleaf *

Vijay Ramani *

Michael Stadler *

Andrew B. Stergachis *

DOI：10.1038/s41580-026-01017-4

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单分子基因组学（SMG）通过实现对单个DNA分子全基因组范围内调控因子活性的表征，改变了我们研究调控基因组机制的能力。SMG能够量化分子异质性和调控事件的共发生，包括表观遗传修饰、转录因子结合和染色质组织在单个DNA分子上的情况。SMG揭示了传统基因组学方法无法测量的染色质相互作用动力学。因此，SMG提供了一个独特的平台来研究调控事件如何协同控制基因组活性。在这篇专家建议文章中，我们提供了一份采用SMG的实用指南，并概述了最佳实践。单分子基因组学方法用于研究单个DNA分子全基因组范围内调控因子的活性，从而实现对分子异质性和调控事件共发生的量化。这篇专家建议文章提供了一份采用单分子基因组学的实用指南，并概述了最佳实践。

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Nature Reviews Molecular Cell Biology

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