A sequence-specific RNA acetylation catalyst

Supuni Thalalla Gamage

doi:10.1093/nar/gkaf217

2025-04-11

Supuni Thalalla Gamage

Shereen Howpay Manage

Manini S. Penikalapati

Jane Jones

Lakshminarayan M. Iyer

DOI：10.1093/nar/gkaf217

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摘要

En 中文

N4-乙酰胞苷（ac4C）是一种由胞苷乙酰转移酶酶类整合的普遍存在的RNA修饰。在此，我们报告了古菌耐热性相关的RNA乙酰转移酶Thermococcus kodakarensis Nat10（TkNat10）的生物化学表征。我们证明TkNat10的催化活性对T. kodakarensis在高温下的适应性至关重要。与真核同源物不同，TkNat10表现出强大的独立活性，以温度、ATP和乙酰辅酶A依赖的方式修饰多种RNA底物。全转录组分析显示TkNat10优先修饰含有5'-CCG-3'共有序列的非结构化RNA。通过高通量诱变方法，我们确定了TkNat10底物识别的序列和结构决定因素。我们发现TkNat10可通过工程改造以利用丙酰辅酶A，为理解其辅因子特异性提供了见解。最后，我们证明TkNat10可用于RNA寡核苷酸的位点特异性乙酰化，从而能够分析ac4C依赖的RNA-蛋白质相互作用。我们的发现为理解古菌RNA乙酰化建立了一个框架，并为研究ac4C在不同RNA背景下的功能后果提供了新工具。

Keyword:

CYTIDINE ACETYLATION

RIBOSOMAL-RNA

N-4-ACETYLCYTIDINE

ACETYLTRANSFERASE

TRANSCRIPTION

PROTEINS

AFFINITY

YIELDS

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Nucleic Acids Research

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13.1

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weizmann institute of science

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411

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Colorado State University System

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nih national cancer institute (nci)

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