Accelerating Live Single-Cell Signalling Studies

Sam Cooper

doi:10.1016/j.tibtech.2017.01.002

2017-05-01

PRE

Sam Cooper *

Chris Bakal

DOI：10.1016/j.tibtech.2017.01.002

原文链接

原文求助

摘要

En 中文

The dynamics of signalling networks that couple environmental conditions with cellular behaviour can now be characterised in exquisite detail using live single-cell imaging experiments. Recent improvements in our abilities to introduce fluorescent sensors into cells, coupled with advances in pipelines for quantifying and extracting single-cell data, mean that high-throughput systematic analyses of signalling dynamics are becoming possible. In this review, we consider current technologies that are driving progress in the scale and range of such studies. Moreover, we discuss novel approaches that are allowing us to explore how pathways respond to changes in inputs and even predict the fate of a cell based upon its signalling history and state.

Keyword:

GENE-EXPRESSION

QUANTITATIVE-ANALYSIS

OPTOGENETIC CONTROL

OSCILLATORY CONTROL

DYNAMIC PROTEOMICS

POSITIVE FEEDBACK

PARTICLE TRACKING

SOFTWARE TOOLS

PROTEIN

TIME

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Trends in Biotechnology

IF:

14.9

论文数:

3.8K

被引数:

2.0W

机构

university of london

学者数:

21.5W

论文数: 19.7W

被引数: 305

引用论文

Probing cellular processes by long-term live imaging - historic problems and current solutions 通过长期实时成像探测细胞过程-历史问题和当前解决方案

JOURNAL OF CELL SCIENCE

IF3.6

2013-01-01

PREAI

Coutu, Daniel L.; Schroeder, Timm

In Critically Ill Patients, Serum Procalcitonin Is More Useful in Differentiating between Sepsis and SIRS than CRP, Il-6, or LBP

Critical Care Research and Practice

IF0

2011-01-01

OAAI

Iwan A. Meynaar; Wouter Droog; Manou Batstra; Rolf Vreede; Paul Herbrink

Dynamic spatiotemporal expression of LIM genes and cofactors in the embryonic and postnatal cerebral cortex

Developmental Dynamics

IF0

2003-02-06

OAAI

Sarada Bulchand; Lakshmi Subramanian; Shubha Tole

Identification of two previously unrecognized genes (guaA and argC) important for uropathogenesis

Molecular Microbiology

IF0

2003-10-31

PREAI

Thomas A. Russo; Stephen T. Jodush; Jennifer J. Brown; James R. Johnson

Questionnaire on the perceptions of patients about total knee replacement

The Journal of Bone and Joint Surgery. British volume

IF0

1998-01-01

PREAI

J. Dawson; R. Fitzpatrick; D. Murray; A. Carr

Dynamics of the p53-Mdm2 feedback loop in individual cells

NATURE GENETICS

IF31.8

2004-01-18

966

OAAI

Lahav, G; Rosenfeld, N; Sigal, A; Geva-Zatorsky, N; Levine, AJ; Elowitz, MB; Alon, U

BAC TransgeneOmics:: a high-throughput method for exploration of protein function in mammals

NATURE METHODS

IF32.1

2008-04-06

514

OAAI

Poser, Ina; Sarov, Mihail; Hutchins, James R. A.; Heriche, Jean-Karim; Toyoda, Yusuke; Pozniakovsky, Andrei; Weigl, Daniela; Nitzsche, Anja; Hegemann, Bjoern; Bird, Alexander W.; Pelletier, Laurence; Kittler, Ralf; Hua, Sujun; Naumann, Ronald; Augsburg, Martina; Sykora, Martina M.; Hofemeister, Helmut; Zhang, Youming; Nasmyth, Kim; White, Kevin P.; Dietzel, Steffen; Mechtler, Karl; Durbin, Richard; Stewart, A. Francis; Peters, Jan-Michael; Buchholz, Frank; Hyman, Anthony A.

Veno-arterial extracorporeal membrane oxygenation: an overview of different cannulation techniques

Journal of Thoracic Disease

IF0

2016-09-01

OAAI

Carlo Banfi; Matteo Pozzi; Marie-Eve Brunner; Fabio Rigamonti; Nicolas Murith; Damiano Mugnai; Jean-Francois Obadia; Karim Bendjelid; Raphaël Giraud

Postdischarge Phone Calls After Pediatric Hospitalization: An Observational Study

Hospital Pediatrics

IF0

2015-05-01

OAAI

Jonathon Heath; Ria Dancel; John R. Stephens