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Adhesion-Based Cell Sorting Platform Using On-Chip Centrifugation

delete2025-08-29
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PRE
AI
M
Mao Otake
T
Takaaki Abe
Y
Yoshiaki Ukita
H
Hiromi Miyoshi
DOI:10.1039/D5LC00578Gdelete
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摘要

摘要

En 中文
细胞分选是生命科学和医学领域中用于细胞分析时选择和纯化目标细胞类型的重要基础过程。特别是,用于稀有细胞分析的高通量细胞分选技术的需求日益增加。为此,我们开发了一种基于离心力的细胞分选技术,该技术以细胞间的粘附力作为标记。我们构建了一个实时观察装置,用于观察细胞在离心过程中的脱附行为,并识别对细胞分选有效的离心条件,例如旋转速度和应用时间。此外,通过结合对恶性纤维肉瘤和非癌成纤维细胞脱附行为的观察,对使用封闭试剂预涂基质表面以控制细胞粘附性的实用性进行了评估。分析结果表明,焦点粘附的平均大小是影响恶性纤维肉瘤和非癌成纤维细胞离心脱附行为的主导参数。这些发现不仅适用于癌细胞分析,而且作为一种微创、高通量的细胞分选技术,也可应用于再生医学领域。
Keyword:
cell sorting
centrifugal force
focal adhesion
rare cells
high-throughput technology

期刊

L
Lab on a Chip
IF:
5.4
论文数:
9.0K
被引数:
3.3W

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