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An upconversion biosensor based on inner filter effect for dual-role recognition of sulfadimethoxine in aquatic samples
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DOI：10.1016/j.foodchem.2023.137832.png)
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Abstract
En 中文
Sulfadimethoxine (SDM) as an extensively employed veterinary drug causes potential threats to human health. Herein, a dual recognition mode novel upconversion fluorescence biosensor was designed based on inner filter effect (IFE) to sensitively and rapidly detect SDM in aquatic samples. Aldehyde-functionalized magnetic nano-particles (MNPs) were applied to recognize and capture SDM, followed by specifically bond with biotin-labeled aptamers. The upconversion nanoparticles and the colored products resulting from the enzyme-catalyzed oxidation of 3,3,5,5-tetramethylbenzidine exhibited an IFE quenching process. Under the optimal condition, the results displayed the fluorescence intensity was correlated with the concentration of SDM within the range of 0.5-1000 ng center dot mL-1 achieving a low limit of detection of 0.13 ng center dot mL-1. The SDM detection system was further employed in the spiked aquatic samples with good recoveries (88.41-96.78 %). Consequently, the constructed fluorescence biosensor provided broad prospects for accuracy and rapid detection of SDM.
Keywords:
Upconversion fluorescence
Sulfadimethoxine
Aptamer
Magnetic separation
Inner filter effect
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9.8
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4.6W
Citations:
24.4W
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