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Assessing crosstalk in simultaneous multicolor single-molecule localization microscopy
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DOI：10.1016/j.crmeth.2023.100571.png)
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摘要
En 中文
MOTIVATION Single-molecule localization microscopy (SMLM) is a straightforward approach to bypass the diffraction limit of optical microscopy and resolve the arrangement of cellular components in their native environment thanks to its <50 nm resolution. However, SMLM acquisitions are slow, particularly for multi-color experiments where channels are usually acquired in sequence. Here we take advantage of a two -cam-era module to evaluate the performance of simultaneous two-color SMLM approaches based on spectrally distinct fluorophores or spectral demixing, focusing on the rigorous assessment of the crosstalk between channels.
Keyword:
FLUORESCENCE NANOSCOPY
SUPERRESOLUTION MICROSCOPY
FLUOROPHORES
ARCHITECTURE
PROTEINS
PLATFORM
PAINT
STORM
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论文数:
954
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2.0K
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