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Axial Plane Optical Microscopy
DOI:10.1038/srep07253.png)
摘要
En 中文
We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.
Keyword:
MECHANOTRANSDUCTION
DIFFRACTION
TOMOGRAPHY
EMBRYOS
DEEP
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期刊
IF:
3.9
论文数:
28.3W
被引数:
83.5W
机构
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