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Cadherin interaction probed by atomic force microscopy
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DOI：10.1073/pnas.070052697.png)
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摘要
En 中文
Single molecule atomic force microscopy was used to characterize structure, binding strength (unbinding force), and binding kinetics of a classical cadherin, vascular endothelial (VE)-cadherin, secreted by transfected Chinese hamster ovary cells as cis-dimerized full-length external domain fused to Fc-portion of human IgC, In physiological buffer, the external domain of VE-cadherin dimers is a approximate to 20-nm-long rod-shaped molecule that collapses and dissociates into monomers (V-shaped structures) in the absence of Ca2+ Trans-interaction of dimers is a low-affinity reaction (K-D = 10(-3-)10(-5) M, k(off) = 1.8 s(-1), k(on) = 10(3)-10(5) M-1 s(-1)) with relatively low unbinding force (35-55 pN at retrace velocities of 200-4,000 nm.s(-1)), Higher order unbinding forces, that increase with interaction time, indicate association of cadherins into complexes with cumulative binding strength. These observations favor a model by which the inherently weak unit binding strength and affinity of cadherin trans-interaction requires clustering and cytoskeletal immobilization for amplification. Binding is regulated by low-affinity Ca2+ binding sites (KD = 1.15 mM) with high cooperativity (Hill coefficient of 5.04), Local changes of free extracellular Ca2+ in the narrow intercellular space may be of physiological importance to facilitate rapid remodeling of intercellular adhesion and communication.
Keyword:
cell adhesion
VE-cadherin
binding strength
binding kinetics
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9.1
论文数:
10.8W
被引数:
73.5W
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